Fig. 1.
Induction of expression of the osteopontin, eosinophil cationic protein, and B144 genes in MEL cells after overexpression of PU.1.
Total RNA was extracted from parental MEL-B8/3 cells (lanes 1-4), mock cells (lanes 5-8), and PU.1-1 cells (lanes 9-12) cultured for 48 hours without reagents (lanes 1, 5, and 9), with DMSO (lanes 2, 6, and 10), with ZnCl2 (lanes 3, 7, and 11), or with DMSO and ZnCl2 (lanes 4, 8, and 12). Expression of the osteopontin, eosinophil cationic protein, and B144 genes was examined by Northern blot analysis.
