Induction of morphologic and functional changes in MEL cells after overexpression of PU.1.
(A) PU.1-1 cells cultured for 48 hours without reagents (upper panel), with ZnCl2 (middle panel), or with ZnCl2 and GM-CSF (1 ng/mL; lower panel) are shown. Arrowheads indicate the adherent cells (spindle shape). (B) NBT reduction activity was measured in PU.1-1 cells, PU.1-2 cells, and mock cells cultured for 48 hours with (▨) or without (■) ZnCl2. Data represent the mean ± SD values from 3 independent experiments. (C) PU.1-1 cells were cultured for 48 hours with ZnCl2, and this was followed by incubation with latex beads for 2.5 hours. Experiments were performed in triplicate. Representative cells taking up latex beads after treatment with ZnCl2 are shown.
