Enforced expression of PKR-sensitized FANCC^−/− cells to IFN-γ and dsRNA-induced apoptosis.

(A) Retrovirally expressed human PKR proteins in MEFs: control cells carrying vector alone (VEC) or cells expressing wild-type human PKR (PKRwt) or PKRΔ6 were treated with or without IFN-γ (10 ng/mL) or dsRNA (100 μg/mL), lysed, and analyzed by Western blotting by using a polyclonal antibody that reacted with both human PKR (hPKR) and mouse PKR (mPKR) proteins. NS, nonspecific. (B) Cell viability of MEFs carrying vector alone (VEC) or expressing wild-type hPKR (wtPKR) or PKRΔ6, in response to IFN-γ or/and dsRNA treatments; data represent the means (standard deviations of triplicate determinations. ▧, FANCC^−/−; ■, FANCC^+/−.