Reciprocal immunoprecipitations.

(A) The reciprocal immunoprecipitations with anti-CD9 (lane 2), anti-αIIb (lane 3), anti-β3 (lane 4), and anti-α6 (lane5) were compared to nonimmune mouse IgG (lane 6) and anti-CD36 antibody FA6-152 (lane 1). Anti-CD9, β3 and α6 antibodies precipitated similar proteins to the anti-CD36 antibody FA6-152. (B) Immunoprecipitated samples were subjected to Western blot analysis with anti-CD36 antibody. Note that 10 μg anti-αIIb antibody was used in the immunoprecipitation to make the CD36 more readily detectable.