American Society of Hematology

Fig. 4.

$Fig. 4. Sucrose gradient ultracentrifugation of detergent extracts. / (A) Biotinylated blood platelets were solubilized in 1% Triton X-100 (A, top) or Brij 99 (A, bottom) and centrifuged on 5% to 40% sucrose step gradients (see “Materials and methods”). An equal volume (10 μL) of each fraction was subjected to SDS-PAGE. Whereas the majority of biotinylated proteins is associated with the high-density factions, a cholesterol-rich population centered on fraction 4 is observed in the lower-density fractions. (B) The presence of the various CD36-associated proteins in fraction 4 from the Triton X-100–solubilized platelets (lane 1) or the Brij 99–solubilized platelets (lane 2) was detected by Western blotting. (C) The distribution of CD36 within the sucrose gradients was determined by Western blotting with an anti-CD36 antibody.$

Sucrose gradient ultracentrifugation of detergent extracts.

(A) Biotinylated blood platelets were solubilized in 1% Triton X-100 (A, top) or Brij 99 (A, bottom) and centrifuged on 5% to 40% sucrose step gradients (see “Materials and methods”). An equal volume (10 μL) of each fraction was subjected to SDS-PAGE. Whereas the majority of biotinylated proteins is associated with the high-density factions, a cholesterol-rich population centered on fraction 4 is observed in the lower-density fractions. (B) The presence of the various CD36-associated proteins in fraction 4 from the Triton X-100–solubilized platelets (lane 1) or the Brij 99–solubilized platelets (lane 2) was detected by Western blotting. (C) The distribution of CD36 within the sucrose gradients was determined by Western blotting with an anti-CD36 antibody.

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