Confocal immunofluorescence visualization of CD36 and α6 (A-D) or CD36 and CD9 (E-H) colocalization in human platelets.

Fixed human platelets were simultaneously incubated with mouse anti-CD36 and rabbit anti-α6 antibodies (A-D) or rabbit anti-CD36 and mouse anti-CD9 (E-H) antibodies, followed by incubation with multiple reporter antibodies. CD36 visualized in the green channel (A,E), colocalized with α6 integrin (B) or CD9 (F), visualized in the red channel giving rise to yellow-orange color in the merged panels (C,G). The fluorograms generated from the red and green intensities within the merged panel are shown in panels D and H. The axes represent red and green pixel intensities (0-255) in the merged panels (C and G).