Confocal immunofluorescence visualization of CD36 and α6 (A-D) or CD9 and α6 (E-H) capping on human platelets.

Fresh platelets were plated on fibronectin-coated coverslips and incubated with mouse anti-CD36 and rat anti-α6 antibodies (A-D) or mouse anti-CD9 and rat anti-α6 antibodies (E-H) at 20°C for 1 hour. α6 was visualized in the green channel (A,E) and CD36 (B) or CD9 (F) was visualized in the red channel. Capping is apparent in the merged panels (C,G) as yellow-orange color and in the fluorograms (D,H). The platelets on fibronectin in this figure are approximately twice as large as those shown in Figure 6 on polylysine.