Fig. 1.
Human serum inhibits TNF-α and IL-1β production in PHA-stimulated PBMC.
PBMC (4 × 105 cells/200 μL/well) were stimulated with 1 μg/mL PHA in medium completed with either FCS or HS. (A) TNF-α and IL-1β were measured in supernatant after 48 hours of incubation; and (B) proliferation (3H-thymidine incorporation) was measured after 72 hours.