American Society of Hematology

Fig. 4.

$Fig. 4. Presence of the inhibitory activity in protein fraction of HDL. / HS was fractionated by high-density centrifugation and the inhibitory activity of HDL, LDL, and serum protein fractions was analyzed. Isolated HDL were further subjected to either delipidation (delipidated HDL) or proteolytic digestion with proteinase K (proteinase K–treated HDL). The inhibitory activity of fractions was compared to HS (whole serum). The final protein concentration for whole serum and serum proteins was 7 mg/mL (black columns), 3.5 mg/mL (hatched columns), and 0.7 mg/mL (white columns). The final protein concentration for LDL was 0.04 mg/mL (black columns), 0.02 mg/mL (hatched columns), and 0.004 mg/mL (white columns). HDL and delipidated HDL was 0.2 mg/mL (black columns), 0.1 mg/mL (hatched columns), and 0.02 mg/mL (white columns). The amount of proteinase K–treated HDL was estimated according to the protein concentration before proteolysis and was similar to untreated HDL. Results are expressed as percentage, 100% being the production of IL-1β or TNF-α measured in the absence of inhibitor (mean ± SD, n = 3).$

Presence of the inhibitory activity in protein fraction of HDL.

HS was fractionated by high-density centrifugation and the inhibitory activity of HDL, LDL, and serum protein fractions was analyzed. Isolated HDL were further subjected to either delipidation (delipidated HDL) or proteolytic digestion with proteinase K (proteinase K–treated HDL). The inhibitory activity of fractions was compared to HS (whole serum). The final protein concentration for whole serum and serum proteins was 7 mg/mL (black columns), 3.5 mg/mL (hatched columns), and 0.7 mg/mL (white columns). The final protein concentration for LDL was 0.04 mg/mL (black columns), 0.02 mg/mL (hatched columns), and 0.004 mg/mL (white columns). HDL and delipidated HDL was 0.2 mg/mL (black columns), 0.1 mg/mL (hatched columns), and 0.02 mg/mL (white columns). The amount of proteinase K–treated HDL was estimated according to the protein concentration before proteolysis and was similar to untreated HDL. Results are expressed as percentage, 100% being the production of IL-1β or TNF-α measured in the absence of inhibitor (mean ± SD, n = 3).

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