Apo A-I decreases the steady-state levels of TNF-α and IL-1β mRNA.
(A,B) Autoradiogram of RNAse protection assay. (A) THP-1 cells (5 × 106 cell/mL) untreated (a) or activated by membranes of stimulated HUT cells (200 μg protein/mL) during 3 hours (b-e) in the presence or absence of apo A-I (200 μg/mL) (c-e), which was added at different time points of THP-1 activation: c (0 hours); d (1 hour); and e (2 hours). (B) Monocytes (10 × 106cell/ml) untreated (a) or activated by membranes of stimulated T lymphocytes (40 μg protein/mL) during 1 hour (b-e) in the presence or absence of apo A-I (200 μg/mL) (c-e), which was added at different time points of THP-1 activation: c (0 minute); d (15 minutes); and e (30 minutes). (C,D) Densitometric analysis of autoradiography A and B, respectively, normalized with the densitometry of GAPDH mRNA = 1, and expressed as percentage, 100% being the mRNA level of activated THP-1 cells (C) or monocytes (D) in the absence of inhibitor. TNF-α (white columns); IL-1β (hatched columns). Results are representative of 3 different experiments.
