CASP2L/AS transfection delays anti-Fas Ab–mediated apoptosis in Jurkat and CEM cell lines.

(A) Western blot analysis of procaspase-2L in Jurkat (Jurkat/AS1 and Jurkat/AS2) and CEM (CEM/AS) mixed cell populations compared to those transfected with the empty vector (Jurkat/neo and CEM/neo). (B, C) Jurkat and CEM cells, transfected with either the empty vector (black bars) or the CASP2L/ASvector (hatched bars) were exposed for 3 hours to indicated concentrations of CH11 Ab (B) or to 50 ng/mL and 1 μg/mL CH11 Ab concentrations, respectively, for indicated times (C). Apoptosis was determined by Hoechst 33342 staining (mean ± SD of 3 independent experiments).