Western blot analyses in Jurkat cells exposed to anti-Fas Abs.

Jurkat and CEM cells were exposed for indicated times to 50 ng/mL and 1 μg/mL CH11 Abs, respectively. (A) DEVDase and IETDase activities were measured in Jurkat/neo (●), Jurkat/AS1 (○), Jurkat/AS2 (■), CEM/neo (●), and CEM/AS (○) as initial velocities. Results were related to those obtained in untreated cells (one representative of 2 experiments; SD, samples in triplicate). (B) Western blot analysis of indicated proteins in Jurkat/neo and Jurkat/AS cells exposed for indicated times to 50 ng/mL CH11 anti-Fas Abs.