Repertoire analysis in a patient with a partial response to HAART.

CD8⁺ PBMCs were studied for a 30-month period (M0 to M30) for patient T16. Viral replication was never completely suppressed during that period of analysis. Gray areas represent significant CD8 clonal expansions (see “Patients, materials, and methods”) found at M0 that remained present 30 months later. The CDR3 length of the major size peaks detected is indicated (arrow and number of amino acid residues). Graphs represent the intensity of fluorescence in arbitrary units, as a function of the CDR3 length, in amino acids of BV-BC single-strand DNA runoff products. Windows of analysis are centered on expected sizes corresponding to TCR transcripts encoding a 10-residue-long CDR3 region.