Fig. 3.
Fig. 3. Effect of PD98059 on the phosphorylation of p42ERK2. / Purified neutrophils were incubated with either DMSO or 30 μM PD98059 for 30 minutes before stimulation with either (A) 1 μM FMLP, (B) 500 U/mL TNF-α, or (C) 10 ng/mL GM-CSF or FCS diluent for the times indicated. Phosphorylation of p42ERK2 was measured by gel retardation as described in “Materials and methods.” The data shown are from a single experiment that was performed twice with similar results.

Effect of PD98059 on the phosphorylation of p42ERK2.

Purified neutrophils were incubated with either DMSO or 30 μM PD98059 for 30 minutes before stimulation with either (A) 1 μM FMLP, (B) 500 U/mL TNF-α, or (C) 10 ng/mL GM-CSF or FCS diluent for the times indicated. Phosphorylation of p42ERK2 was measured by gel retardation as described in “Materials and methods.” The data shown are from a single experiment that was performed twice with similar results.

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