Effect of MAFP on neutrophil arachidonate release and superoxide production.

Neutrophils were preincubated with MAFP at the range of concentrations indicated in the text, followed by priming with either (A, C) 10 ng/mL GM-CSF (●) or (B, D) 500 U/mL TNF-α (●) or FCS diluent (○) and stimulated with either 1 μM FMLP (○) or PBS (■). (A, B) Arachidonate release was measured as described in “Materials and methods.” Stimulation with FMLP was for 30 minutes. (C, D) Superoxide production was measured by the reduction of cytochrome c in a dual-beam spectrophotometer. Stimulation with FMLP was for 10 minutes.