Expression of receptor transcripts expected to be found in primitive hematopoietic precursors.

Autoradiographs for layout A hierarchy blots probed with c-kit (A, Table 1) and flk-1 are shown above left, and the corresponding signals are arranged on hierarchy trees below left. (right) Global cDNAs from the indicated sources were subjected to target-specific secondary PCR, and the products were run out on agarose gels containing ethidium bromide. Each gel contained molecular weight markers corresponding to the number of bases shown at right. Positive PCR controls are shown at right for flk-1 (globally amplified cDNA from murine aorta) and for flk-2 (globally amplified cDNA from sorted rhodamine 123^loLy6A/E^hi murine marrow cells). C-kit, B primers (Table 1), 35 cycles, 60°C annealing, target fragment size 248 bases; flk-1, 35 cycles, 65°C, target fragment size 248 bases; flk-2/flt-3, 41 cycles, 65°C, target fragment size 265 bases. Below each ethidium gel is the corresponding gel blot hybridized with the corresponding radiolabeled probe.