Receptor transcripts expected to differ among pluripotent precursors of different kinds.

Autoradiographs from type A (IL-7Rα, IL-6Rα, IL-11Rα) and type B blots are shown (top left), and the corresponding arrangements in hierarchy trees are shown beneath them. (right) Results of target-specific secondary PCR amplification, as detailed in the legend to Figure 4. Primers were directed at alternative upstream or downstream IL-7Rα transcript termini, as explained in the footnote to Table 1. Upstream primers, 35 cycles, 67°C annealing, followed by 5 cycles, 60°C annealing, target fragment size 317 bases. Downstream primers, 35 cycles, 67°C, target fragment size 249 bases.