Examples and summary of methylation analysis of multiple CpG islands in AML.

Aberrant methylation was detected by bisulfite-PCR (left of each panel) and quantitated by densitometry, and a scatter plot of all the data in 36 patients is shown on the right of each panel. The genes examined are shown below each panel. After bisulfite modification, DNA was amplified by means of primers that amplify methylated and unmethylated alleles equally. PCR products were then digested with the use of restriction enzymes that cleave methylated alleles only. Methylation bands are indicated by arrows.