Fig. 1.
![Fig. 1. Pulse-chase analysis of [674C] and [674R]GPIIb-IIIa synthesis. / CHO cells, transiently transfected with cDNAs encoding GPIIIa and either normal [674C] or mutant [674R]GPIIb, were pulse-labeled with [35S]methionine for 30 minutes and then chased with medium containing unlabeled methionine. At the indicated times, the cells were lysed and immunoprecipitated with anti-GPIIb. The precipitates were analyzed by electrophoresis as described in “Materials and methods.”](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/9/10.1182_blood.v97.9.2640/6/h80910985001.jpeg?Expires=1735832300&Signature=FpkBBkdkIidgC2a3AADxz7uDfra72n4CIMvrofLsFh~ajNpxK~dULUGUsWDQ7BFvhuZ7MMkZJP9GZGkZSy~MKgIzKz7EFvzUujjI~3FjzGb0MN48LK0P-uXdLJYT-xWik8Klwi1NFOHiXCkzSneSU8W~gitdv9G3D9mEvJqbXHiONQPUH8q8kUJXyjkVVuQtlOGHJ2~bKedc0j1OLah9ipvzX6M4sZ3Y0OCjB03qdDYEiGIvmMoPD32mjX7R4Dbe3rofNXiZX3lzGc7qGbwZx~gnMkMou5AbTOW7cul1NgW-XUGbrzRybepGHC-BKSIQGQQSNZnJRmnXmFifOH5MLg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Pulse-chase analysis of [674C] and [674R]GPIIb-IIIa synthesis.
CHO cells, transiently transfected with cDNAs encoding GPIIIa and either normal [674C] or mutant [674R]GPIIb, were pulse-labeled with [35S]methionine for 30 minutes and then chased with medium containing unlabeled methionine. At the indicated times, the cells were lysed and immunoprecipitated with anti-GPIIb. The precipitates were analyzed by electrophoresis as described in “Materials and methods.”
