Fig. 7.
![Fig. 7. Pulse-chase analysis of cells cotransfected with GPIIIa and either normal GPIIb, mutant [674R]GPIIb, or equimolar amounts of both GPIIb cDNAs. / CHO cells, transiently transfected with cDNAs encoding GPIIIa and either normal [674C]GPIIb, mutant [674R]GPIIb, or equimolar amounts of both cDNAs, were pulse-labeled with [35S]methionine for 30 minutes and then chased with medium containing unlabeled methionine. At the indicated times, the cells were lysed and immunoprecipitated with either anti-GPIIb or anti-GPIIIa. The precipitates were analyzed by electrophoresis as described in “Materials and methods.”](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/9/10.1182_blood.v97.9.2640/6/h80910985007.jpeg?Expires=1735832176&Signature=Toze14S1FZcb5os9-PUJRAXqRQxVQbOB9nHFRg96yn3shlItGjMvcVw~zEoaf41DX~Xc-YF3Scakb3ciN7o3CmSwU3xGL2j0GKSYKRik85lDClGYexygRid4UIrXykkE0RbPIetJDgPAGseqroZDcCyjGB65o406H82ab22oxQN~N0hGbmcxpwnq5thRWXGGKLlGez9QPD8x7CpQFElP~w9o~5HQYhfAkpieNhQIclugDu3ZDYbJ5RYOWDSx3Umkx-7dItxMAg91ne4t3jap9HW5dlUMqFtLQNH8RXJjnN3KLb~-ALMWcz901zzEI4B8bGvPvBT~WKb9UBWWpnUxmw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Pulse-chase analysis of cells cotransfected with GPIIIa and either normal GPIIb, mutant [674R]GPIIb, or equimolar amounts of both GPIIb cDNAs.
CHO cells, transiently transfected with cDNAs encoding GPIIIa and either normal [674C]GPIIb, mutant [674R]GPIIb, or equimolar amounts of both cDNAs, were pulse-labeled with [35S]methionine for 30 minutes and then chased with medium containing unlabeled methionine. At the indicated times, the cells were lysed and immunoprecipitated with either anti-GPIIb or anti-GPIIIa. The precipitates were analyzed by electrophoresis as described in “Materials and methods.”
