Development of transgenic mice expressing human GPA.

(A) Schematic representation of selected BAC clone 159F4 containing the entire glycophorin A gene (GYPA). The insert (121 kb) is shown by a horizontal line between NotI sites in the BAC vector. Closed boxes along the line represent the 7 exons of theGYPA gene (A1-A7), and the open box denotes the 3′-flanking precursor (Pr) genomic segment. STS markers used to screen the human genomic BAC library (STS 2; intron2/exon3) and to confirm the presence of the promoter (STS 1) and precursor (STS 3) are designated by horizontal bars. (B) PCR analysis of offspring of transgenic mice. Genomic DNA isolated from tails of mice 3 weeks of age was tested by PCR for the presence of the GYPA transgene using the following sets of primers: STS 1, STS 2, and STS 3. Human genomic DNA and BAC clone 159F4 DNA were used as positive controls.