Fig. 3.
Fig. 3. Western blot analysis of human and murine GPA expression. / (A) Equivalent loads (4 μg/lane) of human, wild-type mouse, andGYPA transgenic mouse red cell proteins were subjected to electrophoresis in a 10% SDS-PAGE gel and stained with Coomassie brilliant blue. No differences were noted in the major red cell membrane proteins in the transgenic sample when compared with the normal mouse sample. (B) Human, wild-type mouse, and GYPAtransgenic mouse red cell proteins (4 μg/lane), separated on an 8% SDS-PAGE gel, were immunoblotted using monoclonal antibody BRIC 163 against the cytoplasmic domain of human GPA. (C) Human, wild-type mouse, and GYPA transgenic mouse red cell proteins were immunoblotted using monoclonal antibody TER-119 against mouse GPA. MW indicates molecular weight.

Western blot analysis of human and murine GPA expression.

(A) Equivalent loads (4 μg/lane) of human, wild-type mouse, andGYPA transgenic mouse red cell proteins were subjected to electrophoresis in a 10% SDS-PAGE gel and stained with Coomassie brilliant blue. No differences were noted in the major red cell membrane proteins in the transgenic sample when compared with the normal mouse sample. (B) Human, wild-type mouse, and GYPAtransgenic mouse red cell proteins (4 μg/lane), separated on an 8% SDS-PAGE gel, were immunoblotted using monoclonal antibody BRIC 163 against the cytoplasmic domain of human GPA. (C) Human, wild-type mouse, and GYPA transgenic mouse red cell proteins were immunoblotted using monoclonal antibody TER-119 against mouse GPA. MW indicates molecular weight.

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