Western blot analysis of TER-119 antibody specificity.

(A) Wild-type (lane 1) and transgenic mouse (lane 2) red cell proteins were solubilized with increased concentrations of SDS, separated on a 10% SDS-PAGE gel, and immunoblotted using monoclonal antibody TER-119. (B) Wild-type mouse red cell proteins, separated on a 10% SDS-PAGE gel, were immunoblotted using rabbit polyclonal antibody against the cytoplasmic domain of human GPA (lane 1) or monoclonal antibody TER-119 (lane 2). (C) Wild-type mouse and band 3 null mouse red cell proteins were immunoblotted using monoclonal antibody TER-119. MW indicates molecular weight.