Effect of postgrafting administration of G-CSF on Th-cell functional phenotype in HLA-mismatched recipients of hematopoietic transplants.

Shown are IL-4, IL-10, and IL-12Rβ₂–chain gene expression by PHA-derived CD4⁺ clones from patients treated with G-CSF (G-CSF+) compared with patients not treated with G-CSF (G-CSF−). Shown are RT-PCR analyses from one donor, one patient treated with G-CSF, and one patient not treated with G-CSF (both 3 months postgrafting); these are representative of analyses done in 10 donors and in all patients between 2 and 6 months postgrafting. C indicates β-actin–specific, cytokine-specific, or cytokine receptor–specific control; and N, no DNA added to the amplification mix during PCR.