Effect of G-CSF on donor DC phenotype.

Shown is the surface expression of CD1, HLA class II, and CD86 molecules on DCs differentiated from donor monocytes exposed to G-CSF. Donor monocytes were incubated in the presence of G-CSF for 24 hours, washed, subjected to standard DC differentiation conditions, and phenotyped by immunofluorescence. Shaded areas indicate DCs differentiated from G-CSF–treated monocytes; solid lines, control DCs; and broken lines, negative controls.