HFE protein is expressed in WT and C282Y cells.

(A) Cell lysates from WT and C282Y cells were immunoprecipitated with anti-HFE antiserum and identified by Western blotting. HFE bands on membranes were identified by immunoblotting with anti-HFE antiserum, as described in the text. (B) HFE expression at the cell surface differs between WT and C282Y cells. Cell-surface proteins were labeled with biotin and immunoprecipitated with anti-HFE antiserum. Immunoprecipitates were resolved by SDS-PAGE and transferred to nitrocellulose membranes. Biotinylated HFE was identified with Streptavidin-HRP.