Visualization of CK1 on nonpermeabilized EA.hy926 endothelial cells.

EA.hy926 cells were grown on permanox tissue culture chambers, fixed with PLPG, and then treated with anti-GPV20, anti-RRY18, or goat IgG (control). After incubation, the slides were treated with a rabbit antigoat antibody and immunostained by the peroxidase/antiperoxidase method as described in “Materials and methods.” The figure is a representative experiment of 2.