Recognition of CK1 on sections of EA.hy926 endothelial cells.

Five- micrometer sections of a cell suspension fixed with PLPG were incubated with anti-GPV20 (A), anti-RRY18 (B), or goat IgG (C) as described in “Materials and methods.” The bound antibodies were visualized by an FITC-labeled rabbit antigoat antibody. The figure is a representative experiment of 2. Note the arrows in panels A and B point to the placement of membrane antigen.