Fig. 5.
![Fig. 5. Flow cytometry for HUVEC kininogen-binding proteins. / Suspensions of washed, unfixed, and nonpermeabilized HUVECs were incubated for 1 hour at 4°C with control IgG (open curves) (goat IgG [either 62 or 300 μg/mL] or mouse IgG [8 μg/mL]) or immune antibodies (shaded curves) (goat anti-GPV20 [300 μg/mL]), mouse anti-uPAR [8 μg/mlL] antibodies, affinity purified goat anti-RRY18 [62 μg/mL]) or mouse anti-gC1qR (4 μg/ml) antibodies. The binding of these antibodies to these cells was detected with secondary antibodies labeled with FITC. The figure is a representative experiment of 3 experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/8/10.1182_blood.v97.8.2342/5/h80810927005.jpeg?Expires=1735509021&Signature=dBGSgIkRgmh-Mm-vXU~zMgGe9QQDiGtD6l7m2ulDTYShH-7G4-zNFHiTs4ai9jWT2OpmqI-M2HzbeB479KeSpDWcIUgBBosR5pZssOBDAT6OI7f8as6~rhVsIXOPYuyVm8ZiuTt3QQaqB6Ddg5Bj0rspemboGmK1G9BGoUVnZwfyX5mgXr4KwJNVOkuI9wg~iIvJjO8QHOlKJWqkpM~wJlprfJ4gvV2HsuuA1K8eDSooNRGHYbkz0UU~Q8i1QxwMvOVI4Oex~FuA-Ts~~HAMyW0fkXkfEyS22sVXkZZ-v-M-Z8~ZWCV2ljYVFki8XmduAuS4DHILNgUbaMvPnxMpgA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Flow cytometry for HUVEC kininogen-binding proteins.
Suspensions of washed, unfixed, and nonpermeabilized HUVECs were incubated for 1 hour at 4°C with control IgG (open curves) (goat IgG [either 62 or 300 μg/mL] or mouse IgG [8 μg/mL]) or immune antibodies (shaded curves) (goat anti-GPV20 [300 μg/mL]), mouse anti-uPAR [8 μg/mlL] antibodies, affinity purified goat anti-RRY18 [62 μg/mL]) or mouse anti-gC1qR (4 μg/ml) antibodies. The binding of these antibodies to these cells was detected with secondary antibodies labeled with FITC. The figure is a representative experiment of 3 experiments.
