Immunoelectron microscopy for gC1qR antigen on nonpermeabilized EA.hy926 endothelial cells.

Cell suspensions were fixed with PLPG and incubated with anti-gC1qR or nonimmune mouse IgG. (A) The bound anti-gC1qR antibodies were visualized by an immunogold procedure as indicated in “Materials and methods.” Clusters of 10-nm gold particles, indicating the presence of bound anti-gC1qR, are seen segmentally on the endothelial plasma membrane. (B) Incubation of nonimmune IgG with the cells. The figure is a representative experiment of 2.