Colocalization of CK1 and uPAR on HUVECs.

HUVECs grown on microscope slides were fixed with 2% paraformaldehyde and not permeabilized. The panels to this figure are photomicrographs of the laser scanning confocal micrographs. The cells in this figure were doubly labeled with primary and secondary antibodies. Upper left panel represents cells that have been labeled with mouse anti-uPAR (8 μg/mL); upper right panel, mouse IgG. Their detection was performed with a second antibody conjugated with FITC. Middle left panel represents cells that have been labeled with goat anti-GPV20 (200 μg/mL); middle right panel, goat IgG (200 μg/mL). Their detection was performed with a second antibody conjugated with Alexa Fluor. Lower left panel represents detection of the 2 labels on these cells that were treated with both goat anti-GPV20 (200 μg/mL) and mouse anti-uPAR (8 μg/mL); lower right panel is a mixture of goat IgG (200 μg/mL), and mouse IgG (8 μg/mL). Recognition of the antibody binding was performed with the secondary antibodies labeled with Alexa Fluor and FITC simultaneously. The figure is a representative presentation of 3 independent experiments.