Expression of IL-12Rβ1 chains and IFN-γ receptors in various cells.

(A-C) PBMCs from the patient and a healthy subject were stimulated with PHA, cultured for 3 days, washed, and incubated with a monoclonal antibody against IL-12Rβ1 chain (black) or a control IgG-1 monoclonal antibody (0.5β), stained with an FITC-labeled, goat-antimouse IgG, and subjected to flow cytometry. The patient's HTLV-1–immortalized T (TS-1HTLV-1) cells were treated and analyzed similarly. (D-E) PBMCs from the patient and a healthy subject were stained with an IFN-γR1–specific monoclonal antibody and subjected to flow cytometry.