Fig. 8.
Induction of expression of p27kip1 and PTEN by vitamin D3 compounds.
(A) Dose-dependent study of p27kip1 and PTEN expression in HL-60 cells analyzed by Western blot. Cells were either untreated (control) or cultured with 10−9 to 10−7 M of either 1,25(OH)2D3 or Gemini-19-nor for 4 days. GAPDH was used as a loading control. (B) Time course study of p27kip1 and PTEN expression in HL-60 cells studied by Western blot. Cells were either untreated (Control) or cultured with Gemini-19-nor (10−7 M) for 0.5 to 4 days. GAPDH was used as a loading control. The densities of the bands were measured using densitometery. (C) Induction of PTEN expression by TPA and ATRA in HL-60 cells. Cells were either untreated (control) or cultured with either TPA (10−9 M) or ATRA (10−7 M) for 4 days. GAPDH was analyzed as a loading control.