Fig. 4.
Flow cytometric evaluation of the cell-cycle profile and of apoptosis in HEL cells left untreated or treated with IR (1.5 and 15 Gy) alone, TRAIL (1 μg/mL) alone, or a combination of IR followed after 6 hours by TRAIL.
Analysis was performed 24 hours after irradiation. The x-axis shows the DNA content, determined based on fluorescence from PI staining, and the y-axis reflects the relative number of cells. Percentage cells in apoptosis (Apo) or in the G1, S, and G2 + M phases of the cell cycle for each experimental point are reported in Tables 1 and 2. These results are representative of 4 experiments performed in duplicate.
