Surface phenotype of CD34⁺-derived erythroblasts at day 10 of liquid culture.

At day 10, cells obtained in liquid cultures supplemented with the SCF + IL-3 + EPO were phenotypically characterized by staining with PE-conjugated anti–glycophorin A. Negative controls are represented by cells stained with isotype-matched PE-conjugated irrelevant monoclonal antibodies (Irr. Ab). Representative results from 1 of 6 separate experiments are shown.