American Society of Hematology

Fig. 1.

$Fig. 1. SOCS-1 is expressed at all stages of T-cell development. / Thymocytes from CD1 fetal thymic lobes or adult thymus were fractionated according to surface expression of CD25 and CD117 (DNI-DNIV) or CD4 and CD8 (DP and SP). The cDNAs were prepared from total RNA, and serial 3-fold dilutions of cDNAs were analyzed for expression of β-actin and SOCS-1 mRNA by RT-PCR. As control (H2O), RT-PCR reactions lacking template were amplified simultaneously.$

SOCS-1 is expressed at all stages of T-cell development.

Thymocytes from CD1 fetal thymic lobes or adult thymus were fractionated according to surface expression of CD25 and CD117 (DNI-DNIV) or CD4 and CD8 (DP and SP). The cDNAs were prepared from total RNA, and serial 3-fold dilutions of cDNAs were analyzed for expression of β-actin and SOCS-1 mRNA by RT-PCR. As control (H₂O), RT-PCR reactions lacking template were amplified simultaneously.

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