Fig. 1.
![Fig. 1. Mac-1–deficient PMNs efficiently phagocytose and kill Ab-opsonized. / C albicans. Tg mouse PMNs (Mac-1+/− and Mac-1−/−) were incubated with C albicansin the presence of FcαRI-directed BsAb. (A) Phagocytosis was analyzed by light microscopy after 30 minutes. (B) Flow cytometric analysis of FcαRI-mediated C albicans uptake by Tg Mac-1+/− (▪) and Tg Mac-1−/− PMNs (░). Results are expressed as percentage of PMNs that mediated phagocytosis (mean ± SEM [n = 4]). (C) FcαRI-mediated killing of C albicans by Tg PMNs (Mac-1+/− and Mac-1−/−) after 2 hours. Results are mean ± SEM based on 4 individual experiments. Bars indicate 10 μm.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/8/10.1182_blood.v97.8.2478/5/h80810929001.jpeg?Expires=1735840454&Signature=IfNM2nNDjRDitj2HQj3D8pGPMIHv3yPexRZnlq5moldCrvJ03q3xiVDwp~0hNHWXGP5YSUFO488PP3fDyx7mxHABD8XrzY7NLYwPCn01iI3YFcUnRph5WdUgT0c500Dk8vLLa1d3oy9MV~lR6nzfZRCGtV0W0EJTkyZAkbHnUsRVRnDH~nWIkFB6nbyNw~6d7YJJ~XrYSmk8RtyIswSy~llSsRVg-vAuWW9S7GZIMmlJ7FDM~cet8rnzs7aMUYWAQxawq6fiX5yJcZmSwd44entFAwPBfIHdHE6QjGi62tHwrbmf14zVnzP7mrXEI243IE7JXIxteGMWDv5Jx39~aA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Mac-1–deficient PMNs efficiently phagocytose and kill Ab-opsonized
C albicans. Tg mouse PMNs (Mac-1+/− and Mac-1−/−) were incubated with C albicansin the presence of FcαRI-directed BsAb. (A) Phagocytosis was analyzed by light microscopy after 30 minutes. (B) Flow cytometric analysis of FcαRI-mediated C albicans uptake by Tg Mac-1+/− (▪) and Tg Mac-1−/− PMNs (░). Results are expressed as percentage of PMNs that mediated phagocytosis (mean ± SEM [n = 4]). (C) FcαRI-mediated killing of C albicans by Tg PMNs (Mac-1+/− and Mac-1−/−) after 2 hours. Results are mean ± SEM based on 4 individual experiments. Bars indicate 10 μm.
