Fig. 3.
![Fig. 3. PMN binding and spreading on Ab-coated targets. / (A) Numbers of Ntg Mac-1+/− PMNs (■), Tg Mac-1+/− PMNs (■), and Tg Mac-1−/− PMNs (░) bound to BsAb [A77 × 520C9]-opsonized SK-BR-3 cells were determined after 30 and 60 minutes. Results represent mean ± SEM from 3 individual experiments. (B) Tg Mac-1+/− PMNs (left) and Tg Mac-1−/− PMNs (right) were plated on IgA-coated glass slides for 30 minutes and stained for actin. Cell morphology was imaged in x/y and x/z directions (inserts), using confocal microscopy. Bars indicate 15 μm. Experiments were performed at least 4 times, yielding similar results.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/8/10.1182_blood.v97.8.2478/5/h80810929003.jpeg?Expires=1735840275&Signature=SN~1njwXKfRf8xJfUaTcC1IWf4C0lasJ4SqJrfyCD6~bRKfXzSVL6r2rYdo~qITiIJKTEBO~bMdcnsQBqoEXoRLcneT-mIlt5AN~H52aM5R-2loizGkCf~2zyY-tKX~6sMRgVudUk6uVATJsLw68S9Wx9CyrJrUFgrmJekc7EIaSlWynRNRYGCI06B-abY6nN-i06rm~PbwVTK4GYMXBg9kb9fvQrcJJiwxbOQVcVxHoTuB4XwMKZDN46F7UJ1pLAYKA6tmiZH34ziyUIB3Jc42r4464OBs3RQNPeqTyA5MDCjM0ocNl5KRDFBZIclzbwtJTdCHD7y1mG-ZJ1YmrZg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PMN binding and spreading on Ab-coated targets.
(A) Numbers of Ntg Mac-1+/− PMNs (■), Tg Mac-1+/− PMNs (■), and Tg Mac-1−/− PMNs (░) bound to BsAb [A77 × 520C9]-opsonized SK-BR-3 cells were determined after 30 and 60 minutes. Results represent mean ± SEM from 3 individual experiments. (B) Tg Mac-1+/− PMNs (left) and Tg Mac-1−/− PMNs (right) were plated on IgA-coated glass slides for 30 minutes and stained for actin. Cell morphology was imaged in x/y and x/z directions (inserts), using confocal microscopy. Bars indicate 15 μm. Experiments were performed at least 4 times, yielding similar results.
