bpV-mediated induction of HIV-1 LTR is sensitive to NaSal, FK506, and CsA.

(A) 1G5 cells were either left untreated (■) or pretreated with NaSal (2.5 mM) (░), FK506 (10 ng/mL) (▪), CsA (100 ng/mL) (▥), or a combination of NaSal with either FK506 (▤) or CsA () for 1 hour at 37°C. Following treatment, cells were either left untreated or treated for 8 hours with the following agents: PHA (3 μg/mL), bpV[bipy], bpV[HO pic], or bpV[pic] (10 μM). Jurkat cells were transiently transfected by the DEAE-Dextran protocol with 15 μg of either pκB-TATA-LUC (B) or pmκBLTR-LUC (C). After 24 hours, transfected cells were either left untreated (■) or were pretreated with FK506 (10 ng/mL) (▪) for 1 hour at 37°C. Following treatment, cells were stimulated for 8 hours with the following agents: PHA (3 μg/mL), PHA (3 μg/mL)/PMA (20 ng/mL), or bpV[pic] (10 μM). Cells were then lysed and evaluated for luciferase activity as described in “Materials and methods.” Values are the mean of 3 different measured samples ± SD. This is representative of 3 independent experiments.