D835 mutant FLT3 transformed 32D cells.

The 32D cells were transfected with D835 mutant cDNA. Stably mutant FLT3-expressing cells were cloned and the surface expression was analyzed by flow cytometer. Expression levels were the same among transfectants (A). Stably mutant FLT3-expressing cells were cultured without FL and IL-3. Mutant FLT3-expressing cells showed autonomous proliferation, as well as FLT3/ITD-expressing cells (B). Furthermore, the proliferation rates of mutant FLT3-expressing cells were the same as those with IL-3 (C).