Cytokines stimulate survivin protein expression in OCI-AML3 cells.

Cells were cultured in serum-free RPMI 1640 medium with GM-CSF (150 U/mL), G-CSF (80 ng/mL), or SCF (75 ng/mL). After 48 hours, cells were lysed, and survivin levels were compared with those in untreated cells by Western blot analysis.