LT-HSC from BM and blood of CY/G-CSF–treated animals express cyclin D2 mRNA.

(A) Splenic T cells, (B) CY/G-CSF day +4 LT-HSC from BM, and (C) CY/G-CSF day +4 MPB LT-HSC. Cells were double sorted into lysis buffer in 96-well plates at 5 cells/well, followed by RT and nested PCR for cyclin D2 (35 cycles per primer pair; see “Materials and methods”). PCR products of the predicted length (331 bp) were obtained. Reverse transcriptase (RT) was omitted from samples in lanes 11 and 12.