Development of Th2 cells from splenocytes is decreased in DO10⁺ T cell receptor transgenic Stat5a^−/−mice.

Splenocytes from DO10⁺ mice (A,C,E) or DO10⁺Stat5a^−/− mice (B,D,F) were stimulated with OVA323-339 peptide (0.1 μg/mL) for 48 hours in Th0- (A,B), Th1- (C,D), or Th2- (E,F) polarizing condition. Cells were cultured in the presence of IL-2 for another 3 days and then restimulated with platebound anti-CD3 mAb for 6 hours. Intracellular cytokine profiles for IL-4 versus IFN-γ were determined on CD4⁺ T cells as described in “Materials and methods.” Shown are representative FACS profiles from 5 mice in each group.