American Society of Hematology

Fig. 10.

$Fig. 10. Depletion of CD4+CD25+T cells increases Th1 cell differentiation but decreases Th2 cell differentiation. / CD4+CD25+ T cells were depleted from splenocytes of DO10+ mice and DO10+Stat5a−/− mice by magnetic cell sorting as described in “Materials and methods.” CD4+CD25+ T cell–depleted or unfractionated splenocytes were stimulated with OVA323-339 peptide (0.1 μg/mL) for 48 hours. Cells were cultured with IL-2 for another 3 days and then restimulated with platebound anti-CD3 antibody for 6 hours. Intracellular staining for IL-4 and IFN-γ on CD4+ T cells was then analyzed by FACS. Shown are representative FACS profiles from 5 mice in each group.$

Depletion of CD4⁺CD25⁺T cells increases Th1 cell differentiation but decreases Th2 cell differentiation.

CD4⁺CD25⁺ T cells were depleted from splenocytes of DO10⁺ mice and DO10⁺Stat5a^−/− mice by magnetic cell sorting as described in “Materials and methods.” CD4⁺CD25⁺ T cell–depleted or unfractionated splenocytes were stimulated with OVA323-339 peptide (0.1 μg/mL) for 48 hours. Cells were cultured with IL-2 for another 3 days and then restimulated with platebound anti-CD3 antibody for 6 hours. Intracellular staining for IL-4 and IFN-γ on CD4⁺ T cells was then analyzed by FACS. Shown are representative FACS profiles from 5 mice in each group.

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