American Society of Hematology

Fig. 8.

$Fig. 8. Fibrinogen inhibits ADP-evoked cytoskeletal association of p60src. / Dimethyl BAPTA-loaded human platelets were stimulated for 3 minutes with no addition or with ADP (5 μg/mL) in the absence or presence of fibrinogen (1 mg/mL) and then lysed with a Triton X-100 buffer and centrifuged to separate the detergent-insoluble fraction. These samples were subjected to SDS–PAGE and analyzed by Western blotting with the specific anti-p60src antibody GD11, as described in “Materials and methods.” Bands were revealed using chemiluminescence and were quantified by scanning densitometry. (Top) Results from a representative experiment. (arrow) Position of p60src. (Bottom) Data are means ± SE of 4 independent experiments and are expressed as -fold increases over the resting levels (experimental/control).$

Fibrinogen inhibits ADP-evoked cytoskeletal association of p60^src.

Dimethyl BAPTA-loaded human platelets were stimulated for 3 minutes with no addition or with ADP (5 μg/mL) in the absence or presence of fibrinogen (1 mg/mL) and then lysed with a Triton X-100 buffer and centrifuged to separate the detergent-insoluble fraction. These samples were subjected to SDS–PAGE and analyzed by Western blotting with the specific anti-p60^src antibody GD11, as described in “Materials and methods.” Bands were revealed using chemiluminescence and were quantified by scanning densitometry. (Top) Results from a representative experiment. (arrow) Position of p60^src. (Bottom) Data are means ± SE of 4 independent experiments and are expressed as -fold increases over the resting levels (experimental/control).

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