Flow cytometric analysis of DCs in the bone marrow aspirate.

A sample of bone marrow harvested from a healthy donor was stained separately with 3 panels of monoclonal antibodies—a combination of CD3– fluorescein isothiocyanate (FITC), CD4-PE, CD8-PerCP, and CD45-APC (A-C); a combination of “lineage” cocktail (CD3, CD14, CD20, CD56)-FITC, CD123-PE, HLA-DR-PerCP, and CD4-APC (D-F); or Lin cocktail-FITC, CD4-PE, HLA-DR-PerCP, and CD11c-APC (G-I). Location of the CD3⁻, CD4^bright, low side-scatter cells is shown by the oval drawn in panels B, E, and H. CD3⁻, CD4^bright, low side-scatter cells are shown as bold black dots (0.14% of nucleated cells) in panels A to C; Lin⁻, CD123^bright, HLADR⁺ cells are shown as bold black dots (0.15% of nucleated cells) in panels D to F; Lin⁻, HLADR⁺, CD11c⁺ cells are shown as bold black dots (1.1% of nucleated cells) in panels G to I.