American Society of Hematology

Fig. 1.

$Fig. 1. Engraftment of human primary T-ALL cells with or without cord blood preconditioning. / Mice were irradiated and injected with or without 10 × 106 cord blood MNCs, 9 days before injection with 2.3 × 106 primary T-ALL cells. They were killed 40 days after T-ALL injection. Bone marrow (A) and spleen (B) cells were analyzed for several phenotypic markers by flow cytometry, as described in “Materials and methods.” For each panel, the filled histogram curve corresponds to the indicated experimental mAb and is superimposed over an open histogram curve corresponding to the isotype control mAb. The fraction of cells staining positively for the experimental mAb was determined by subtraction of the curves, using CellQuest 3.2.1 software. Percentages of human CD45+, CD7+, and CD19+ cells are indicated in the panels.$

Engraftment of human primary T-ALL cells with or without cord blood preconditioning.

Mice were irradiated and injected with or without 10 × 10⁶ cord blood MNCs, 9 days before injection with 2.3 × 10⁶ primary T-ALL cells. They were killed 40 days after T-ALL injection. Bone marrow (A) and spleen (B) cells were analyzed for several phenotypic markers by flow cytometry, as described in “Materials and methods.” For each panel, the filled histogram curve corresponds to the indicated experimental mAb and is superimposed over an open histogram curve corresponding to the isotype control mAb. The fraction of cells staining positively for the experimental mAb was determined by subtraction of the curves, using CellQuest 3.2.1 software. Percentages of human CD45⁺, CD7⁺, and CD19⁺ cells are indicated in the panels.

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