Coexpression pattern of c/ebp1 andl-plastin in zebrafish embryos.

RNA in situ hybridization was performed using a digoxigenin-labeled c/ebp1 RNA antisense probe developed with BM purple, and a fluorescein-labeled l-plastin RNA antisense probe developed with fast red. Lateral views of a tail region immediately caudal to the yolk sac extension oriented with the head to the left are shown. In the upper panel a bright field view of an in situ hybridized 24-hpf embryo shows l-plastin staining in red and c/ebp1 staining in purple. The lower panel shows the same view with l-plastin staining visualized with a rhodamine filter under fluorescence, photographed with a Quantix CCD camera, and pictured with red pseudocoloring using IP lab software. The blue arrow shows cells expressing both c/ebp1 andl-plastin. The yellow arrow indicates a cell with undetectable expression of c/ebp1, but strong expression ofl-plastin. The white arrow shows a cell with high expression of c/ebp1, but minimal l-plastinexpression.