Fig. 4.
Luciferase assay in MST cells that are spontaneously expressing the
NDST-2 gene. (A) Luciferase activity under the control of promoter region and 5′-UTR of the NDST-2 gene in MST cells. (B) A part of 5′-UTR of the NDST-2 gene between +649 and +668. Two GGAA motifs are underlined. (C) Luciferase activity of the reporter plasmid containing the intact GGAA motifs or the motifs mutated at either 5′-GGAA or 3′-GGAA sequence. The data represent the mean ± SE of 3 experiments. In some cases, the SE was too small to be shown by bars. *P < .01 by t test when compared with the luciferase activity obtained by the transfection of the reporter plasmid containing −196 to +668 region.