Coimmunoprecipitation of MITF and GABP.

Myc-tagged +-MITF or Myc-tagged mi-MITF was cotransfected to COS-7 cells with GABPα (A) or GABPβ (B). The whole cell extract was subjected to immunoprecipitation with anti- GABPα or GABPβ Ab. Precipitates were separated by SDS-PAGE and immunoblotted with anti-Myc Ab.